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ppcr-script cam+ cloning vector  (Agilent technologies)


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    Structured Review

    Agilent technologies ppcr-script cam+ cloning vector
    Ppcr Script Cam+ Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcr+script+vector/pm37834049-458-2-6
    Average 90 stars, based on 1 article reviews
    ppcr-script cam+ cloning vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response.
    Article Snippet: Neutrophils were purified from human blood using Lymphoprep (Stemcell Technologies, Fisher Scientific, Seattle, WA, USA) and CD4+ and CD8+ T cells were purified from human blood using magnetic beads (MACS Technology, Milteny Biotceh, San Diego, CA, USA).

    Construct:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Amplification:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Clone Assay:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.

    Plasmid Preparation:

    Article Title: Trypanosoma brucei rhodesiense Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response
    Article Snippet: .. To generate the ICP re-expression construct, the ICP gene was amplified from the genomic DNA with primers NT90/NT91 [ ] using Pfu Turbo polymerase and cloned into the pPCR Script vector (Stratagene). .. After confirming the sequence, the gene was excised from the sub-cloning vector using Xho I/ Xba I and ligated into a similarly digested plasmid containing a phleomycin resistance cassette to generate the plasmid pGL1493.



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